动漫爱好者形成了一种独特的亚文化，他们有自己的俚语和聚会活动。

Anime fans have developed their own ___subculture with unique slang and gathering events.

为了进一步研究，科学家们将细胞在新鲜培养基中进行了继代培养。

The scientists ___subcultured the cells in fresh medium for further study.